Category: Moms

Antifungal activity of plant extracts

Antifungal activity of plant extracts

Trichophyton rubrum. Actiity mecanismos de atividade antifúngica de óleos Antiffungal Soccer nutrition for professional players fungos patogênicos. We found activitu studies have previously selected plants based extracfs these indications and have observed anti-microbial or anti-fungal properties in these plants. The concentration tested ranged from 20 to 0. Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh,India. Indian J Med Res — Anyone you share the following link with will be able to read this content:. Antifungal activity of plant extracts

Video

Antimicrobial Properties Of Plant Extract - Investigation Of Antimicrobial Activity Of Plant -

Antifungal activity of plant extracts -

Open Access. Issue E3S Web Conf. Current usage metrics About article metrics Return to article. Initial download of the metrics may take a while.

Table of Contents. Next article. Article contents Abstract PDF 2. Database links NASA ADS Abstract Service. Metrics Show article metrics. Services Articles citing this article CrossRef 1. Related Articles Antifungal activity of Moroccan plants against citrus fruit pathogens Agron.

Bookmarking Mendeley. Reader's services Email-alert. E3S Web Conf. The plant material was collected in an area of Caatinga, located in the municipality of Altinho Pernambuco, NE Brazil. The exsiccates of the collected plants were identified by experts and deposited in the herbaria of the Instituto Agronômico de Pernambuco IPA.

The plant material parts of plants used medicinally, as indicated in the database was collected from at least three individuals of each species and shade dried at room temperature.

Successive extractions were performed until complete extraction of the plant material. The first one was performed after 48 hours and the others at hour intervals. After this period, the solvent was removed using the rotary evaporator at a temperature of 40 °C.

The obtained extract was placed in a desiccator. The extracts were tested against C. albicans ATCC , C. neoformans ATCC , and C. gattii ATCC obtained from the Laboratório de Diversidade Molecular da Universidade Federal de Alagoas UFAL.

In vitro susceptibility of yeast isolates was performed using broth microdilutions according to the methodology recommended by the Clinical and Laboratory Standards Institute - CLSI in MA3 protocol Clinical and Laboratory Standards Institute - CLSI. CLSI document MA3. Philadelphia, Wayne.

An inoculum was prepared by suspension of colonies in saline solution 0. The crude extracts were resuspended in dimethyl sulfoxide DMSO in a ratio of The concentration tested ranged from 20 to 0.

The microdilution plates containing RPMI RPMI tissue culture medium supplemented with glutamine buffered to pH 7. Following this, the plates were incubated at 35 °C for h. The positive control was composed of culture medium and yeast, and the negative control contained DMSO in the concentration used to dilute the extracts.

As antifungal control, we used two agents of different classes: Amphotericin B and fluconazole, with the concentrations tested ranging from 16 to 0. Antifungal susceptibility of Cryptococcus neoformans to amphotericin B and fluconazole.

and the CLSI manual. For determining whether the extracts used present fungicidal or fungistatic activity, a small volume 5 μL of each of the wells with no apparent yeast growth were inoculated in YEPD agar medium and incubated at 35 °C for 48 hours.

To avoid antifungal carryover, aliquots were deposited as a spot onto the agar plate and allowed to soak. The result was obtained according to the formation, or not, of colonies at the inoculated site.

These were included as control strains in each set of experiments. Among the 30 plants selected for the three groups of criteria mentioned, only 23 were tested due to difficulties in availability, since the Caatinga environment presents a strong seasonality, which limits the temporal supply of plant material to few months of the year Tab.

Thumbnail Table 2 Plant species selected based on different selection criteria for antifungal evaluation. According to the value of the SIV, eight species were indicated as priority see Tab.

The species that were calculated to possess the highest weight were M. urundeuva and A. Both had a higher frequency of citation and weight of the symptoms compared to those of the other species.

Although the number of active extracts did not differ with respect to the selection criteria, it was possible to observe divergence among them with respect to the degree of inhibitory activity and the number of strains susceptible to the extracts.

For example, plants cited as anti-inflammatory and selected by SIV were seen to be more effective against C. neoformans alone. The proportion of active plants has demonstrated the relevance of the ethnodirected approaches to test the in vitro activity of crude vegetal extracts against fungi.

Studies have confirmed that plants which are reported to be used by local populations have higher antimicrobial potential than those which are selected by other approaches, such as random selection. For example, Svetaz et al. found that the probability of finding plants with anti-fungal properties was higher in those with ethnomedical uses related to fungal infections compared to those that were randomly selected.

The plants used for indications of infections and inflammation showed interesting results against the analyzed fungi. We found that studies have previously selected plants based on these indications and have observed anti-microbial or anti-fungal properties in these plants. A study implemented in Chile has verified the antifungal action of plants which were used for injuries and associated infections against Penicillium expansum and C.

Among the 40 evaluated species, 30 presented interesting antimicrobial activities, corroborating with their traditional uses Silva et al. Braga et al. selected plants traditionally used in infectious diseases and inflammation and evaluated their activity against fungi. Among the 24 methanolic extracts obtained from 20 plants, only those of Schinus terebintifolius , O.

gratissimum, Cajanus cajan , and Piper aduncum , with MIC of 1. In contrast, the species Bixa Orellana, O. gratissimum and Syzygium cumini with MIC of 0. The proportion of species with interesting activities has been lower than that observed in our studies.

However, the definition of the criteria has been important in the attempt to reduce efforts and costs with in vitro tests.

From the total number of extracts evaluated 23 , ten extracts showed activity against C. The inhibitory activity of the extracts against C. gattii was verified if the extracts exhibited activity against C.

albicans and C. Of the plants prioritized by the SIV, only five A. occidentale, M. urundeuva, P. pyramidalis, A. colubrina var. cebil, and M. oftalmocentra presented antifungal effects against all three strains C.

albicans, C. neoformans, and C. gattii , with MIC ranging from 0. The extracts that showed strong inhibitory activity were A. occidentale bark extract for C. neoformans , compared to fluconazole, and extracts of M. urundeuva and P. pyramidalis , compared to amphotericin B, against the same strain Tab.

Among the eight randomly selected plants with citations for use in inflammation, extracts of L. ferrea , S. brasiliensis, and P. granatum showed fungicidal action against all strains, with MIC between 0.

The extract from the bark of S. brasiliensis showed strong fungicidal activity for C. neoformans MIC 0. Among the seven medicinal plants randomly selected used in cases without indications of inflammation and infection , only E.

pyriformis extract showed fungistatic activity against C. neoformans MIC of 0. The hydroalcoholic extracts from B. cheilantha and C. tapia were the only ones considered inactive against the three strains Tab.

However, for this same selection category, most of the extracts reported were inactive against only C. albicans Tab. A previous study Cruz et al. Antifungal activity of Brazilian medicinal plants involved in popular treatment of mycoses. evaluated the activity of Z. joazeiro, Caesalpinia pyramidalis valid name: Poincianella pyramidalis , Bumelia sartorum valid name : Sideroxylon obtusifolium , and Hymenaea courbaril , which are plants popularly known for their treatment of mycoses, against C.

guilliermondii, C. neoformans, and Trichophyton rubrum. Of these, only the aqueous extracts obtained from the leaves of C. pyramidalis and from the bark of Z.

joazeiro were effective MIC of 6. guilliermondii and T. Similar to our results, Z. joazeiro showed substantial activity against C. However, no activity was reported against C.

albicans and Z. joazeiro presented the best activity in the study carried out by Cruz et al. Finding plants with antifungal potential has not been easy Souza Souza NAB. Possíveis mecanismos de atividade antifúngica de óleos essenciais contra fungos patogênicos.

PhD Thesis. Universidade Federal da Paraíba, João Pessoa. Due to this complexity concerning the bioprospecting of plants with antifungal activity, our data show that the use of direct citations for infections and inflammations may be a good tool in the search of potential antifungal candidates, since medicinal plants without these indications did not present better activity.

The data used to support the findings of this study are included within the article and can be solicited by request to the authors. To the members of Laboratório de Ecologia e Evolução de Sistemas Socioecológicos da Universidade Federal de Pernambuco and to the Laboratório de Microbiologia e Biologia Molecular da Universidade Regional do Cariri URCA for their support.

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil CAPES , Finance Code , with contributions from the INCT Ethnobiology, Bioprospecting, and Nature Conservation certified by CNPq, and financial support from FACEPE Grant number: APQ Open menu Brazil.

Acta Botanica Brasilica. Submission of manuscripts About the journal Editorial Board Instructions to authors Contact.

Português Español. Open menu. table of contents « previous current next ». Text EN Text English. PDF Download PDF English. ABSTRACT Plants have been reported as used by local populations to treat various infections for a long time, which has directed several pharmacological studies.

Introduction One of the great current challenges in the treatment of fungal diseases has been the resistance they have acquired to certain compounds. Materials and methods Data treatment and plant selection Plants were selected from an ethnobotanical survey executed in a rural community located in the municipality of Altinho, Pernambuco, in northeastern Brazil Silva et al.

Table 1 Weighted p values attributed to each anti-microbial and anti-inflammatory indication attributed to the plants cited in the free list performed in a rural community located in an area of Caatinga, Pernambuco, Brazil. Table 2 Plant species selected based on different selection criteria for antifungal evaluation.

Table 3 Syndromic Importance Value SIV of plants used in cases of inflammation and infections in the area of Caatinga, Northeast Brazil. Acknowledgements To the members of Laboratório de Ecologia e Evolução de Sistemas Socioecológicos da Universidade Federal de Pernambuco and to the Laboratório de Microbiologia e Biologia Molecular da Universidade Regional do Cariri URCA for their support.

References Ahmed AS, McGaw LJ, Elgorashi EE, Naidoo V, Eloff JN. Araújo TAS, Alencar NL, Amorim ELC, Albuquerque UP. Ayres M, Ayres MJ, Ayres DL, Santos SA.

Bastos GM, Nogueira NAP, Soares CL, Martins MR, Rocha LQ, Teixeira AB. Biasi-Garbin RP, Demitto FO, Amaral RCR, et al Braga FG, Bouzada MLM, Fabri RL, et al

abbad or. Fungal diseases have always been a plany problem for tomato Optimal pre-workout meals. Growers generally use chemical extacts Antifungal activity of plant extracts treat this type of diseases. However, these atcivity are Antifungal activity of plant extracts to the environment and the consumer, especially if the pre-harvest interval is not respected. The present study aims to find non-polluting alternatives. Five plant extracts Peganum harmala, Ocimum basilicum, Caralluma europaea, Nerium oleander and Eucalyptus globulus are tested for their in vitro efficiency against four pathogenic fungi: Alternaria solani, Botrytis cinerea, Botrytis cinerea taken from fruit, Phytophthora infestans and Oïdium oxysporum. The obtained results reveal that the extract of Ocimum basilicum is the most effective on the studied fungi. Today, Soccer nutrition for professional players fungi, especially Candida albicans, are among the most common risk factors olant patients Antifungla weakened immune systems. Fungal Amazon Fashion Trends caused by Candida species and increasing Antifungal activity of plant extracts Antiffungal azole-resistant strains are of Antifuntal importance in Antifungzl patients. The toxicity of the drugs used, the development of resistance to these fungi, and the problems caused by drug interactions necessitate the use of more effective drugs with less toxicity. This study aimed to evaluate the antifungal activity of medicinal plant extracts on C. In this review study, Scopus, PubMed, MEDLINE, Google Scholar, SID, and Magiran databases were searched between and to identify related articles. All in vivo and in vitro trials were included in the study. The reviewed studies showed the effectiveness of some compounds in inhibiting the growth of C.

Author: Tokus

1 thoughts on “Antifungal activity of plant extracts

Leave a comment

Yours email will be published. Important fields a marked *

Design by ThemesDNA.com